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KMID : 0665220110240010065
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Korean Journal of Food and Nutrition
2011 Volume.24 No. 1 p.65 ~ p.70
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Effects of a Corn Extract on Mouse Splenocyte and Cytokine Production by Peritoneal Macrophages
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Ryu Hye-Sook
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Abstract
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This study was performed to investigate the in vitro effect of a corn water extract on immune function. Splenocyte proliferation was determined by the MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl terazolium bromide) assay after preparing asingle cell suspension. Production of macrophage-secreted interleukin(IL)-, IL-6, and interferon(IFN)-, was detected by ELISA using a cytokine assay kit. After a 48-hr incubation with mitogens(ConA or lipopolysaccharide), mice splenocyte proliferation increased with the addition of a corn water extract supplement at 10, 50, 100, 250, 500, or . Production of IL-, IL-6, and IFN- increased in treatments supplemented with the corn water extract. In an in vitro study, splenocyte proliferation increased when corn water extract was added. In an ex vivo experiment, the highest production of cytokines by activated peritoneal macrophages was observed in mice orally administered 500 mg/kg body weight/day.
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KEYWORD
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splenocyte proliferation, corn, cytokine, IL-, IL-6, IFN-, immunity
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